The dorsoventral polarity of Drosophila egg is established by means of signaling between the germ line-derived oocyte and the surrounding somatic follicle cells.
Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.
The present study is the first to report on the usefulness of puromycin for production of enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer.
The process of somatic cell nuclear transfer involves removing genetic material from the nucleus of a host egg cell and replacing it with the nucleus from adult cells.